The science behind the only microalgae of its kind.

Peer-reviewed mechanistic research. Third-party tested by Eurofins every lot. Single origin. Fully traceable.

Not all microalgae are equal.

Spirulina is a cyanobacterium. Chlorella is a green alga. Both have evolved entirely in aquatic environments. Klebsormidium flaccidum is something categorically different.

Classified as a Charophyte — the same evolutionary lineage that gave rise to all terrestrial plants — Klebsormidium sits at the transition point between aquatic algae and land plants. This dual heritage produces a compound profile that neither kingdom can replicate alone: the bioactive complexity of a land plant combined with the bioavailability of a microalgae.

ZL01 is the specific patented strain cultivated by Zivolife. It is the only commercially available Klebsormidium flaccidum in the world. Every lot is grown at a single dedicated facility in Ica, Peru, third-party tested by Eurofins to AOAC and USP standards for composition, heavy metals, and microbiology, and imported under an active FDA Foreign Supplier Verification Program certificate.

cGMP Certified Eurofins Tested Every Lot Self-affirmed GRAS

Beta-Glucan

Naturally occurring polysaccharides found in the cell walls of certain algae, grains, and fungi. Beta-glucans are well-studied for their ability to activate innate immune cells including macrophages and NK cells, support gut microbiome balance, and modulate cholesterol metabolism.

Protein (Complete)

A complete amino acid profile providing all nine essential amino acids required for tissue repair, enzyme production, immune function, and sustained metabolic energy. Plant-sourced proteins with high bioavailability contribute meaningfully to daily protein requirements.

Dietary Fiber

Non-starch polysaccharides that promote gut motility, support the growth of beneficial microbiota, aid in nutrient absorption, and may contribute to cardiovascular health through cholesterol-lowering mechanisms.

ALA (Omega-3)

Alpha-linolenic acid is a plant-derived polyunsaturated omega-3 fatty acid and the dominant PUFA in Zivolife's lipid profile. Omega-3 fatty acids support cardiovascular health by reducing triglycerides and inflammation, enhance brain function and mood, and promote joint comfort.

Iron

An essential mineral required for hemoglobin synthesis and oxygen transport throughout the body. Adequate iron levels are directly linked to energy production, cognitive performance, and immune competency.

Vitamin K₁

Phylloquinone (K₁) is the plant form of Vitamin K, essential for blood coagulation, bone mineralization, and the inhibition of arterial calcification. It activates matrix Gla protein (MGP), a key regulator of vascular elasticity and calcium handling.

Vitamin E

A fat-soluble antioxidant that neutralizes free radicals within cell membranes, protecting lipids from oxidative damage. Vitamin E also supports immune response and plays a role in skin barrier integrity and tissue repair.

Vitamin A (β-Carotene)

Beta-carotene is a provitamin A carotenoid converted to retinol in the body as needed — a self-regulating mechanism that avoids the toxicity risks associated with preformed vitamin A. It supports epithelial integrity, visual function, and immune modulation.

Lutein

A xanthophyll carotenoid that accumulates in the macula of the eye, where it functions as a natural blue-light filter and antioxidant shield. Lutein is among the best-characterized compounds for long-term visual health and retinal protection.

Zeaxanthin

A structural isomer of lutein and co-dominant pigment in the central macula. Zeaxanthin works synergistically with lutein to absorb damaging high-energy visible light and quench reactive oxygen species that accumulate in retinal tissue.

Total Carotenoids

A collective class of lipid-soluble pigments including alpha-carotene, beta-carotene, lutein, zeaxanthin, and related compounds. Carotenoids serve as broad-spectrum antioxidants, quenching singlet oxygen and scavenging peroxyl radicals across multiple tissue compartments.

Polyphenols

A structurally diverse class of plant secondary metabolites with potent antioxidant and anti-inflammatory activity. Polyphenols modulate the Nrf2 pathway — the body's master antioxidant regulator — and support gut microbiome diversity through prebiotic fermentation.

Chlorophyll

Porphyrin-ring pigments responsible for photosynthesis. Chlorophylls and their derivatives have demonstrated binding affinity for heavy metal ions and mutagens, contributing to the body's natural detoxification pathways.

Potassium

The primary intracellular cation, essential for maintaining fluid balance, nerve signal transmission, and muscle contraction — including cardiac rhythm. Potassium acts as a physiological counterbalance to sodium, supporting healthy blood pressure.

Calcium

The most abundant mineral in the human body, with primary structural roles in bone and tooth mineralization. Calcium also serves as a critical intracellular second messenger involved in muscle contraction, nerve impulse transmission, and enzyme activation.

Pheophorbide a

A chlorophyll-derived porphyrin unique to certain microalgae and a notable bioactive in var. ZL01's mechanistic profile. Pheophorbide a has been identified as an iNOS inhibitor with NF-κB modulating activity, placing it within the body's healthy inflammatory response pathway.

The evidence behind the organism.

Peer-reviewed mechanistic research. A 90-day safety study with zero adverse effects.

In vitro mechanistic study

Anti-inflammatory compounds identified by UHPLC-MS analysis.

Researchers at the University of Mississippi's National Center for Natural Products Research used ultrahigh-performance liquid chromatography mass spectrometry to identify the specific anti-inflammatory compounds present in ZL01.

Four classes of bioactive compounds were identified as contributing to anti-inflammatory activity: hydroxylated fatty acids, chlorophyll-derived pheophorbides, carotenoids, and glycoglycerolipids.

Pheophorbide a was identified as the most potent compound. It inhibited inducible nitric oxide synthase (iNOS), a key enzyme in the body's healthy inflammatory response, at a concentration of 0.24 µM. It also demonstrated inhibition of NF-κB, a central transcription factor in inflammatory signaling.

IC50 0.24 µM

Pheophorbide a · iNOS inhibition

In vitro · LPS-induced macrophage model · University of Mississippi, 2020

Qiu, S., Khan, S.I., Wang, M. et al. (2020). Chemometrics-assisted identification of anti-inflammatory compounds from the green alga Klebsormidium flaccidum var. zivo. Molecules, 25(5), 1048. DOI: 10.3390/molecules25051048

90-day subchronic toxicity study

Safety margin exceeds 1,000× typical human exposure.

9.7 g/kg/day

NOAEL, highest dose tested, zero adverse effects

1,000×+

Safety margin vs. typical human exposure

Adverse effects observed at any dose level

A 90-day subchronic oral toxicity study established a No-Observed-Adverse-Effect Level of 9.7 grams per kilogram body weight per day, which was the highest dose tested. No adverse effects were observed at any dose. Genotoxicity testing was negative on both Ames assay and micronucleus assay. No cyanotoxins or harmful byproducts were detected in harvested biomass.

Steffek, A.E. et al. (2018). 90-day dietary toxicity study and genotoxicity studies with Klebsormidium flaccidum var. ZIVO dried algal biomass. Toxicology Reports, 5, 959–969. PMC6169147

One whole food. The evidence is there.

One published human trial. Peer-reviewed mechanistic research. A 90-day safety study with zero adverse effects. Third-party tested by Eurofins every lot. Single origin. Fully traceable.

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Grown at one farm. Tested every lot. Fully traceable.

ZL01 is cultivated exclusively at the Alimenta Algae S.A.C. facility in Ica, Peru — a high-altitude coastal desert environment chosen for its solar intensity, water purity, and isolation from agricultural contamination. The Ica region provides year-round cultivation conditions unavailable in most other geographies.

The facility operates under cGMP certification with HACCP protocols. Low-temperature drying preserves all bioactive compounds and phytonutrients, yielding a 24-month shelf life. No solvents, no preservatives, no co-manufactured ingredients.

Every commercial lot is third-party tested by Eurofins in the USA for full macronutrient and micronutrient composition, heavy metals including lead, mercury, and cadmium, and complete microbiological screening before product release. Full Certificate of Analysis is available for every lot.

Single Origin · Ica, Peru cGMP Certified HACCP Certified Eurofins Tested Every Lot FDA FSVP Validated 2024 Full Lot Traceability